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Home > Product > ELISA Primacu > Influenza A H3N2 Hemagglutinin Primacu™ ELISA Kit

Influenza A H3N2 Hemagglutinin Primacu™ ELISA Kit

Influenza A H3N2 Hemagglutinin Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 125.2 pg/mL

Detection range: 390.63-25000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with:

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 125.2 pg/mL

Detection range: 390.63-25000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with:

Product overview

Full product name

Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin / HA

Code

BPE170

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

125.2 pg/mL

Detection range

390.63-25000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Storage

2-8°C

Assay time

3.5h

Background

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

Research area

Immunology

Target protein

Influenza A H3N2 HA

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE170 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
25000 pg/ml 2.662 2.744 2.635
2.825
12500 pg/ml 1.606 1.604 1.495
1.602
6250 pg/ml 0.836 0.871 0.762
0.905
3125 pg/ml 0.533 0.534 0.425
0.534
1562.5 pg/ml 0.271 0.287 0.178
0.302
781.25 pg/ml 0.2 0.188 0.079
0.175
390.625 pg/ml 0.148 0.155 0.046
0.161
0 pg/ml 0.112 0.109 0
0.105

Precision

We measured random samples of BPE170 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 1551 135 8.7
Intra-assay 2 20 3299 250 7.6
Intra-assay 3 20 13748 1124 8.2
Inter-assay 1 20 1533 111 7.3
Inter-assay 2 20 3322 261 7.9
Inter-assay 3 20 13677 924 6.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE170

    $2320.00

  • 96T

    BPE170

    $580.00

  • 15*96T

    BPE170

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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