Product name
Recombinant Rhesus Macaque TNF-Alpha protein
Code
CD00021
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using Mouse L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.
Endotoxin
Less than 1 EU/μg of rRhTNF-α as determined by LAL method.
Synonyms
TNF-α, TNF-a, TNF-alpha, TNFA, TNFSF2,Monkey
Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL
Uniprot Accession
Molecular Weight
Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Rhesus macaque and human TNF-α show approximately 98 % homology at the amino acid level. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
Storage
-20ºC
Research area
Immunology
