Product name
Recombinant Rhesus Macaque IL-8/CXCL8 protein
Code
CD00032
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected Mouse BaF3 cells is in a concentration range of 0.5-5.0 ng/ml.
Endotoxin
Less than 1 EU/μg of rRhIL-8/CXCL8 as determined by LAL method.
Synonyms
IL-8/CXCL8, IL8,Monkey
Sequence
AVLPRSAKEL RCECIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE PWVQRVVEKF VKRAENQNP
Uniprot Accession
Molecular Weight
Approximately 9.14kD containing 79 amino acid residues.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. Rhesus macaque IL-8 has approximately 94 % amino acid sequence identity with human IL-8.
Storage
-20ºC
Research area
Immunology
