Product name
Recombinant Rhesus Macaque IL-1 Alpha protein
Code
CD00041
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse D10S cells is less than 10 pg/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.
Endotoxin
Less than 0.1 EU/μg of rRhIL-1α as determined by LAL method.
Synonyms
IL-1α, IL1A,Monkey
Sequence
SAPFSFLSNM TYHFIRIIKH EFILNDTLNQ TIIRANDQHL TAAAIHNLDE AVKFDMGAYT SSKDDTKVPV ILRISKTQLY VSAQDEDQPV LLKEMPEINK TITGSETNFL FFWETHGTKN YFISVAHPNL FIATKHDNWV CLAKGLPSIT DFQILENQA
Uniprot Accession
Molecular Weight
Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar identical biological properties. Among various species, the amino acid sequence of mature IL-1α is conserved 60 % to 70 %. IL-1α recently started to find effective application in cosmetic and dermatological formulations, which allow to significantly harmonizing derma architecture.
Storage
-20ºC
Research area
Others
