Product name
Recombinant Rhesus Macaque Eotaxin/CCL11 protein
Code
CD00049
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml.
Endotoxin
Less than 0.1 EU/μg of rRhEotaxin/CCL11 as determined by LAL method.
Synonyms
Eotaxin/CCL11,Eotaxin, SCYA11, Monkey
Sequence
GPDSVATTCC FTLTNKKIPL QRLESYRRII SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDRKSP TPKP
Uniprot Accession
Molecular Weight
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 6.9.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Rhesus Macaque CCL11 is belonging to the CC chemokine family. It is encoded by the gene CCL11. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Rhesus macaque CCL11 is approximately 90 % identical at the amino acid level to human CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to monkey MCP-1. CCR3 has been identified to be a specific CCL11 receptor.
Storage
-20ºC
Research area
Immunology
