Product name
Recombinant Rat SCF protein
Code
CD00076
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse MC/9-2 mast cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
Endotoxin
Less than 1 EU/μg of rRtSCF as determined by LAL method.
Synonyms
KITLG, Kitl, Mgf, SCF,Rat
Sequence
MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Uniprot Accession
Molecular Weight
Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. In addition, it also promotes mast cell adhesion, migration, proliferation, and survival 3. Rat SCF shares 75 % - 90 % a.a. sequence identity with canine, feline, mouse, and human SCF. Furthermore, rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells.
Storage
-20ºC
Research area
Immunology
