Product name
Recombinant Rat MIP-1 Beta/CCL4 protein
Code
CD00086
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.
Endotoxin
Less than 1 EU/μg of rRtMIP-1β/CCL4 as determined by LAL method.
Synonyms
MIP-1β/CCL4,Mip1-b, Scya4, Rat
Sequence
APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN
Uniprot Accession
Molecular Weight
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 3 % trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Chemokine (C-C motif) ligand 4 encoded by the CCL4 gene, also known as macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant rat CCL4 contains 69 amino acids and it shares 80 % and 86 % a.a. sequence identity with human and Mouse CCL4. Both human and Mouse MIP-1α and MIP-1β are active on human and Mouse hematopoietic cells.
Storage
-20ºC
Research area
Immunology
