Product name
Recombinant Rat MIP-1 Alpha/CCL3 protein
Code
CD00087
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human blood monocytes is in a concentration range of 10-100 ng/ml.
Endotoxin
Less than 0.1 EU/μg of rRtMIP-1α/CCL3 as determined by LAL method.
Synonyms
MIP-1α/CCL3, MIP-1a, Scya3Rat
Sequence
APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA
Uniprot Accession
Molecular Weight
Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Chemokine (C-C motif) ligand 3 encoded by the CCL3 gene, also known as macrophage inflammatory protein-1α (MIP-1α) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. Recombinant CCL3 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant rat CCL3 contains 69 amino acids and it shares 72 % and 91 % a.a. sequence identity with human and Mouse CCL3. Both human and Mouse MIP-1α and MIP-1β are active on human and Mouse hematopoietic cells.
Storage
-20ºC
Research area
Others
