Product name
Recombinant Rat Midkine protein
Code
CD00089
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.
Endotoxin
Less than 0.1 EU/μg of rRtMidkine as determined by LAL method.
Synonyms
MDK,ARAP, MK, NEGF2,Midkine, Rat
Sequence
VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD
Uniprot Accession
Molecular Weight
Approximately 13.2 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family and it is encoded by the MDK gene. Midkine includes five intrachain disulfide bonds which held two domains and there are three antiparallel beta-sheets in each domain. A chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta (PTPzeta), is a receptor for MK. MK promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The predicted molecular weight is approximately 13.2 kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Mature rat midkine shares 89% and 98% a.a. sequence identity with human and Mouse.
Storage
-20ºC
Research area
Immunology
