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Home > Product > Recombinant proteins > Recombinant Rat IL-33 protein

Recombinant Rat IL-33 protein

Recombinant Rat IL-33 protein

Product Summary

Size: 10 ug,2 ug

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product Summary

Size: 10 ug,2 ug

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product overview

Product name

Recombinant Rat IL-33 protein

Code

CD00104

Biological activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

Endotoxin

Less than 1 EU/μg of rRtIL-33 as determined by LAL method.

Synonyms

IL-33,IL33,RGD1311155, Rat

Sequence

SIQGTSLLTE SCALSTYNDQ SVSFVLENGC YVINVEDCGK NQEKDKVLLR YYESSFPAQS GDGVDGKKLM VNMSPIKDTD IWLNANDKDY SVELQKGDVS PPDQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE ENDESCNNIM FKLSKM

Uniprot Accession

Molecular Weight

Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Background

Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a cytokine belonging to the IL-1 superfamily. It is also a proinflammatory protein that may regulate gene transcription and it induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL-33 is constitutively expressed in smooth muscle and airway epithelia and it binds to a high-affinity receptor family member ST2. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues. Recombinant rat IL-3 contains 156 amino acid residues and it shares 59 % a.a. and 90 % sequence identity with human and Mouse IL-33.

Storage

-20ºC

Research area

Immunology

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Associated products

  • Size

    Code

    Price

  • 10 ug

    CD00104

    $409.00

  • 2 ug

    CD00104

    $176.00

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Lead time: Within two weeks

We are continuously updating our Recombinant proteins catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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