Product name
Recombinant Rat IL-3 Beta protein
Code
CD00105
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse MC-9 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
Endotoxin
Less than 1 EU/μg of rRtIL-3β as determined by LAL method.
Synonyms
IL-3β, Rat
Sequence
MISDRGSDAH HLLRTLDCRT IALEILVKLP YPQVSGLNNS DDKANLRNST LRRVNLDEFL KSQEEFDSQD TTDIKSKLQK LKCCIPAAAS DSVLPGVYNK DLDDFKKKLR FYVIHLKDLQ PVSVSRPPQP TSSSDNFRPM TVEC
Uniprot Accession
Molecular Weight
Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated Mouse mast cells, Mouse keratinocytes and neurons/astrocytes. It exerts its biological activities through binding to Interleukin-3 receptors included α and β subunits, but sequence analysis revealed a number of features indicative of the presence of only one β-subunit in the rat. Interleukin-3 acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Furthermore, rat and human IL-3 share low homology and have not cross species activity.
Storage
-20ºC
Research area
Immunology
