Product name
Recombinant Rat IL-1 Alpha protein
Code
CD00114
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse D10S cells is less than 0.005 ng/ml, corresponding to a specific activity of > 2.0 × 108 IU/mg.
Endotoxin
Less than 1 EU/μg of rRtIL-1α as determined by LAL method.
Synonyms
IL-1α,IL-1 alpha, IL-1F1,IL1A, Rat
Sequence
SAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDDSKYP VTLKVSNTQL FVSAQGEDKP VLLKEIPETP KLITGSETDL IFFWEKINSK NYFTSAAFPE LLIATKEQSQ VHLARGLPSM IDFQIS
Uniprot Accession
Molecular Weight
Approximately 17.8 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 8.5.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. Recombinant rat IL-1 alpha contains 150 amino acids which is a single non-glycosylated polypeptide.
Storage
-20ºC
Research area
Immunology
