Product name
Recombinant Rat HB-EGF protein
Code
CD00118
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
Endotoxin
Less than 1 EU/μg of rRtHB-EGF as determined by LAL method.
Synonyms
HB-EGF, Dtr, GFHB, Hbegf, Hegfl,Rat
Sequence
DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL
Uniprot Accession
Molecular Weight
Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 300 mM NaCl, pH 7.4, 5 % trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. In addition, it shares about 73 % and 76 % a.a. sequence identity with Mouse and rat HB-EGF.
Storage
-20ºC
Research area
Immunology
