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Home > Product > Recombinant proteins > Recombinant Rat GM-CSF protein

Recombinant Rat GM-CSF protein

Recombinant Rat GM-CSF protein

Product Summary

Size: 5 ug

Purity: > 98 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product Summary

Size: 5 ug

Purity: > 98 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product overview

Product name

Recombinant Rat GM-CSF protein

Code

CD00121

Biological activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse FDC-P1 is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.

Endotoxin

Less than 1 EU/μg of rRtGM-CSF as determined by LAL method.

Synonyms

GM-CSF,Gm-csf, Gmcsf,CSF2, Rat

Sequence

APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQK

Uniprot Accession

Molecular Weight

Approximately 14.5 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Background

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. It is reported that GM-CSF has no biological effects across species.

Storage

-20ºC

Research area

Immunology

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Associated products

  • Size

    Code

    Price

  • 5 ug

    CD00121

    $176.00

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Lead time: Within two weeks

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