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Home > Product > Recombinant proteins > Recombinant Rat FGF-21 protein

Recombinant Rat FGF-21 protein

Recombinant Rat FGF-21 protein

Product Summary

Size: 5 ug

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product Summary

Size: 5 ug

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product overview

Product name

Recombinant Rat FGF-21 protein

Code

CD00126

Biological activity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 103 IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 10 μg/ml of heparin.

Endotoxin

Less than 1EU/μg of rRtFGF-21 as determined by LAL method.

Synonyms

FGF-21, Fgf8c,FGF21,Rat

Sequence

AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS

Uniprot Accession

Molecular Weight

Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 500 mM NaCl, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Background

Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81 % and 92 % a.a. identity to human and Mouse FGF-21, and it show activity on Mouse and human cells.

Storage

-20ºC

Research area

Immunology

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Associated products

  • Size

    Code

    Price

  • 5 ug

    CD00126

    $176.00

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Lead time: Within two weeks

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