Product name
Recombinant Rat Eotaxin/CCL11 protein
Code
CD00129
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 0.1-1.0 μg/ml.
Endotoxin
Less than 1 EU/μg of rRtEotaxin/CCL11 as determined by LAL method.
Synonyms
Eotaxin/CCL11,Scya11, Rat
Sequence
HPGSIPTSCC FTMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTKLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
Uniprot Accession
Molecular Weight
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
CCL11 encoded by the gene CCL11is belonging to the CC chemokine family. It is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. CCL11 is a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. CCR3 has been identified to be a specific CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.
Storage
-20ºC
Research area
Immunology
