Product name
Recombinant Rat CNTF protein
Code
CD00133
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg.
Endotoxin
Less than 1 EU/μg of rRtCNTF as determined by LAL method.
Synonyms
CNTF, Rat
Sequence
AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
Uniprot Accession
Molecular Weight
Approximately 22.7 kDa, a single non-glycosylated polypeptide chain containing 199 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 2 % trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Recombinant rat CNTF containing 199 amino acids and it shares 83 % and 95 % a.a. sequence identity with human and Mouse CNTF.
Storage
-20ºC
Research area
Immunology
