Product name
Recombinant Rat CDNF protein
Code
CD00137
Biological activity
Fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 - 25 μg/mL on a nitrocellulose-coated microplate.
Endotoxin
Less than 0.1 EU/μg of rRtCDNF as determined by LAL method.
Synonyms
CDNF, Armetl1,Rat
Sequence
QGLEAGVRSR ADCEVCKEFL NRFYNSLLTR GIDFSVDTIE EELISFCADT KGKENRLCYY LGATKDSATK ILGEVTRPMS VHMPTVKICE KLKKMDSQIC ELKYEKKLDL ESVDLWKMRV AELKQILHSW GEECRACAEK HDYVNLIKEL APKYVETRPQ TEL
Uniprot Accession
Molecular Weight
Approximately 18.8 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Cerebral dopamine neurotrophic factor (CDNF), also known as ARMET-like protein 1, is encoded by the CDNF gene and widely expressed in neuronal and non-neuronal tissues. The CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). Furthermore, it also prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. By research, CDNF might be beneficial for the treatment of Parkinsons disease. Recombinant rat CDNF contains 163 amino acid residues and it shares 84 % and 87 % a.a. sequence identity with human and Mouse CDNF.
Storage
-20ºC
Research area
Immunology
