Product name
Recombinant Rat C10/CCL6 protein
Code
CD00140
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CCR1 transfected Mouse BaF3 cells is in a concentration range of 10-100 ng/ml.
Endotoxin
Less than 1 EU/μg of rRtC10/CCL6 as determined by LAL method.
Synonyms
C10/CCL6, Mrp-1, Scay6,Rat
Sequence
GLIQDTVKED RPFNPTIIHQ GFQDSSDCCF SYASQIPCSR FIYYFPTSGG CTKPGIIFVT RKRKRVCANP SDQRVQTCIS TLKLGPRSGN SAIA
Uniprot Accession
Molecular Weight
Approximately 10.4 kDa, a single non-glycosylated polypeptide chain containing 94 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Chemokine (C-C motif) ligand 6 (CCL6), encoded by the gene CCL6, is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It signals primarily through the CCR1 receptor. CCL6 is chemotactic for B cells, CD4+ T cells, monocytes and NK cells and also exhibits powerful suppressive activity on colony formation by different lineages of hematopoietic progenitors. It contains the four highly conserved cysteine residues present in CC chemokines. The completed rat CCL6 sequence is 115 amino acids (a.a.) containing 21 a.a. signal peptide. It has about 78 % identity with mouse homologue (116 a.a.).
Storage
-20ºC
Research area
Immunology
