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Product > Recombinant proteins > Recombinant Protein A/G (C-6His)
Recombinant Protein A/G (C-6His)
Recombinant Protein A/G (C-6His)
Product Summary
Size:
1 mg
Purity: > 96 % by SDS-PAGE and RP-HPLC analyses.
Source: Escherichia coli.
Product Summary
Size:
1 mg
Purity: > 96 % by SDS-PAGE and RP-HPLC analyses.
Source: Escherichia coli.
Product overview
Product name
Recombinant Protein A/G (C-6His)
Code
CD00148
Endotoxin
Less than 0.1 EU/μg of Protein A/G, His as determined by LAL method.
Synonyms
Protein A/G, His
Molecular Weight
Approximately 56.9 kDa with 6×His-tag on C-terminus, a single non-glycosylated polypeptide chain containing 513 amino acids.
Formulation
Lyophilized with no additive.
Reconstitution
Dissolve in distilled water or saline.
Background
Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5-8.
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