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Home > Product > Recombinant proteins > Recombinant Porcine IL-1RA protein

Recombinant Porcine IL-1RA protein

Recombinant Porcine IL-1RA protein

Product Summary

Size: 5 ug

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product Summary

Size: 5 ug

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product overview

Product name

Recombinant Porcine IL-1RA protein

Code

CD00154

Biological activity

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of Mouse D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 75 pg/ml rPoIL-1α.

Endotoxin

Less than 0.1 EU/μg of rPoIL-1RA as determined by LAL method.

Synonyms

IL-1RA,IRAP1, IL1RN,Porcine

Sequence

HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ

Uniprot Accession

Molecular Weight

Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Background

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the co-receptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The porcine IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Porcine IL-1RA shares 86 % and 87 % a.a. sequence homology with bovine and canine IL-1RA.

Storage

-20ºC

Research area

Immunology

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Associated products

  • Size

    Code

    Price

  • 5 ug

    CD00154

    $176.00

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Lead time: Within two weeks

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