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Home > Product > Recombinant proteins > Recombinant Mouse VEGF165 protein

Recombinant Mouse VEGF165 protein

Recombinant Mouse VEGF165 protein

Product Summary

Size: 10 ug,2 ug

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product Summary

Size: 10 ug,2 ug

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product overview

Product name

Recombinant Mouse VEGF165 protein

Code

CD00160

Biological activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.

Endotoxin

Less than 1 EU/μg of rMuVEGF165 as determined by LAL method.

Synonyms

VEGF165, Mouse

Sequence

MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Uniprot Accession

Molecular Weight

Approximately 38.8 kDa, a disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Background

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGFs normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Mouse and rat express alternately spliced isoforms of 120, 165, and 188 amino acids (a.a.) in length. Recombinant mouse VEGF165 contains 165 amino acids residues and it is a disulfide-linked homodimer. In addition, it shares 97 % a.a. sequence identity with corresponding regions of rat, 89 % with human and porcine, 88 % with bovine, and 90 % with feline, equine and canine VEGF, respectively.

Storage

-20ºC

Research area

Immunology

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Associated products

  • Size

    Code

    Price

  • 10 ug

    CD00160

    $409.00

  • 2 ug

    CD00160

    $176.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our Recombinant proteins catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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