Product name
Recombinant Mouse MIP-3 Alpha/CCL20 protein
Code
CD00294
Biological activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR6 transfected Mouse BaF3 cells is in a concentration range of 0.1-10 ng/ml.
Endotoxin
Less than 1 EU/μg of rMuMIP-3α/CCL20 as determined by LAL method.
Synonyms
MIP-3α/CCL20, CKb4, LARC, MI, MIP, MIP-3, MIP-3A, MIP-3[a], MIP3A, ST3, ST38, Scya, Scya20, exodus, exodus-1,Mouse
Sequence
ASNYDCCLSY IQTPLPSRAI VGFTRQMADE ACDINAIIFH TKKRKSVCAD PKQNWVKRAV NLLSLRVKKM
Uniprot Accession
Molecular Weight
Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
CCL20, also named LARC (Liver and Activation-regulated Chemokine), is encoded by CCL20 located on chromosome 1 in mouse. The expression can be induced by LPS and some inflammatory cytokines, and it is at a high level in lymph nodes, PBL, fatal liver, fatal lung, appendix and so on. CCL20 has functions of chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. CCL20 elicits it effects by binding with CCR6 receptor.
Storage
-20ºC
Research area
Immunology
