Product name
Recombinant Mouse MIP-2/CXCL2 protein
Code
CD00295
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 1.0-10 ng/ml.
Endotoxin
Less than 1 EU/μg of rMuMIP-2/CXCL2 as determined by LAL method.
Synonyms
MIP-2/CXCL2, CINC, CINC-2a, GROb, Gr, Gro2, MIP, MIP-2, MIP-2a, Mgsa, Mgsa-b, Mi, Mip2, Scy, Scyb, Scyb2,Mouse
Sequence
AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKVCLDPEAPLVQ KIIQKILNKG KAN
Uniprot Accession
Molecular Weight
Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Mouse CXCL2, also named MIP-2, is belonging to the CXC chemokine family. It is encoded by the gene CXCL2. CXCL2 shares 90 % amino acid sequence with CXCL1/GROα. It is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is secreted from a Mouse macrophage cell line. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. Similar to other GRO proteins, CXCL2 is potent neutrophil attractants and activators. In addition, it is also active toward basophils. The amino acid sequence of Mouse CXCL2 is 60 % identical to human CXCL2.
Storage
-20ºC
Research area
Immunology
