Product name
Recombinant Mouse MIP-1 Alpha/CCL3 protein
Code
CD00298
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using Mouse splenocytes is in a concentration range of 10-100 ng/ml.
Endotoxin
Less than 0.1 EU/μg of rMuMIP-1α/CCL3 as determined by LAL method.
Synonyms
MIP-1α/CCL3,AI323804, CCL, G0S19-, G0S19-1, LD78, LD78alpha, MIP-, MIP-1alpha, MIP1, MIP1-, MIP1-(a), MIP1-alpha, Mi, Mip1a, Scy, Scya3, Mouse
Sequence
APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA
Uniprot Accession
Molecular Weight
Approximately 7.9 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
CCL3, also known as macrophage inflammatory protein-1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1α can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant Mouse MIP-1α is a 7.9 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and it shares approximately 71 % and 91 % amino acid sequence homology with the human and rat protein.
Storage
-20ºC
Research area
Others
