Product name
Recombinant Mouse MDC/CCL22 protein
Code
CD00304
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human activated lymphocytes is in a concentration range of 10-100 ng/ml.
Endotoxin
Less than 1 EU/μg of rMuMDC/CCL22 as determined by LAL method.
Synonyms
MDC/CCL22, ABCD-, ABCD-1, DCBCK, MDC, Scya, Scya22,Mouse
Sequence
GPYGANVEDS ICCQDYIRHP LPSRLVKEFF WTSKSCRKPG VVLITVKNRD ICADPRQVWV KKLLHKLS
Uniprot Accession
Molecular Weight
Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 68 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
CCL22 is a protein that in mouse is encoded by the CCL22 gene, which locates on the Chr. 8. It is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69).
Storage
-20ºC
Research area
Immunology
