Product name
Recombinant Mouse LIX/CXCL5 protein
Code
CD00321
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.
Endotoxin
Less than 1 EU/μg of rMuLIX/CXCL5 as determined by LAL method.
Synonyms
LIX/CXCL5, AMCF, AMCF-II, Cxcl, Cxcl6, ENA-, ENA-78, GCP-, GCP-2, LIX, Scyb, Scyb5, Scyb6,Mouse
Sequence
APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ
Uniprot Accession
Molecular Weight
Approximately 9.8 kDa, a single, non-glycosylated polypeptide chain containing 92 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
CXCL5 is a small cytokine belonging to the CXC chemokine family and it is encoded by the CXCL5 gene. It May participate in the recruitment of inflammatory cells by injured or infected tissue. CXCL5 is cleaved into the following 2 chains: GCP-2 (1-78) and GCP-2 (9-78) which attract neutrophils and are involved in neutrophil activation. Recombinant Mouse CXCL5 contains 92 amino acids and shares 57 % amino acid sequence identity with human CXCL5.
Storage
-20ºC
Research area
Immunology
