Product name
Recombinant Mouse IL-7 protein
Code
CD00351
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse 2E8 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
Endotoxin
Less than 1 EU/μg of rMuIL-7 as determined by LAL method.
Synonyms
IL-7, A630026I06Rik, Il, Il7, hlb36, hlb368Mouse
Sequence
ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI
Uniprot Accession
Molecular Weight
Approximately 14.9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 2 % trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-7 (IL-7) is encoded by the IL7 gene in mouse and secreted by stromal cells in the red marrow and thymus. The protein signals through the IL-7 receptor, which is a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and it can stimulate proliferation of B cells, T cells and NK cells. Mouse IL-7 has approximately 65 % and 88 % amino acid sequence identity with human and rat IL-7 and both proteins exhibit cross-species activity. IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection.
Storage
-20ºC
Research area
Immunology
