Product name
Recombinant Mouse IL-4 protein
Code
CD00355
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant prolifiration of Mouse HT-2 cells is less then 2 ng/ml, corresponding to a Specific Activity of > 5 × 105 IU/mg.
Endotoxin
Less than 1 EU/μg of rMuIL-4 as determined by LAL method.
Synonyms
IL-4,BSF-1, Il, Il4, Mouse
Sequence
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
Uniprot Accession
Molecular Weight
Approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 121 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It is produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. IL-4 enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The mouse IL-4 has a compact, globular fold, stabilised by 3 disulphide bonds and contains 121 amino acids residues which is a single non-glycosylated polypeptide. The human IL-4 shares about 40 % a.a. sequence identity with mouse/rat IL-4 and they are species-specific in their activities.
Storage
-20ºC
Research area
Immunology
