Product name
Recombinant Mouse IL-36 Alpha
Code
CD00361
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in Mouse NIH/3T3 cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 × 104 IU/mg.
Endotoxin
Less than 1 EU/μg of rMuIL-36α, 153a.a. as determined by LAL method.
Synonyms
IL-36α, IL36A,Fi, Fil, Fil1, IL-1, IL-1H1, IL1RP2, If36a, Il1f6, Il1f9, Mouse
Sequence
RAASPSLRHV QDLSSRVWIL QNNILTAVPR KEQTVPVTIT LLPCQYLDTL ETNRGDPTYM GVQRPMSCLF CTKDGEQPVL QLGEGNIMEM YNKKEPVKAS LFYHKKSGTT STFESAAFPG WFIAVCSKGS CPLILTQELG EIFITDFEMI VVH
Uniprot Accession
Molecular Weight
Approximately 17.1kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, 1 mM DTT, 3 % trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36α is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant Mouse interleukin-36 alpha contains 153 amino acids residues which is a single non-glycosylated polypeptide. Specifically, mouse IL-36α shares 83 % a.a. sequence identity with rat IL-36α, 54 – 60 % with human, rabbit, equine and bovine IL-36α.
Storage
-20ºC
Research area
Immunology
