Product name
Recombinant Mouse IL-1 Beta protein
Code
CD00395
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 108 IU/mg.
Endotoxin
Less than 1EU/μg of rMuIL-1β as determined by LAL method.
Synonyms
IL-1β, IL-, IL-1beta, Il-1b,IL1B,Mouse
Sequence
VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS
Uniprot Accession
Molecular Weight
Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
Background
Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. The mature mouse IL1β shares 90 % amino acid sequence identity with cotton rat and rat and 65 %-78 % identity with canine, human and rhesus IL1β.
Storage
-20ºC
Research area
Immunology
