Product name
Recombinant Mouse IFN- Lambda3/IL-28B protein
Code
CD00407
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg.
Endotoxin
Less than 1 EU/μg of rMuIFN-λ3/IL-28B as determined by LAL method.
Synonyms
IFN-λ3/IL-28B,Il; IFL; Il2; Il28; IFL-1; INF-l; Il28b; IL-28B; If1ia2; INF-alpha; INF-lambda,Ifnl3, Mouse
Sequence
DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKGAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENINDS ALTTILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFQLL LRDLKCVASG DQCV
Uniprot Accession
Molecular Weight
Approximately 19.6 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Mouse IFN-lambda 2 (IL-28A), IFN-lambda 3 (IL-28B) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid sequence identity) and type I IFN family (15 - 19% a.a. sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28B shares 63%, 63%, and 52% a.a. identity with human IL-28A, IL-28B, and IL-29, respectively.
Storage
-20ºC
Research area
Immunology
