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Home > Product > Recombinant proteins > Recombinant Mouse IFN- Gamma protein

Recombinant Mouse IFN- Gamma protein

Recombinant Mouse IFN- Gamma protein

Product Summary

Size: 100 ug,20 ug

Purity: > 96 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product Summary

Size: 100 ug,20 ug

Purity: > 96 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product overview

Product name

Recombinant Mouse IFN- Gamma protein

Code

CD00410

Biological activity

Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using Mouse L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8 ng/ml, corresponding to a specific activity of > 1.3 × 106 IU/mg.

Endotoxin

Less than 1 EU/μg of rMuIFN-γ as determined by LAL method.

Synonyms

IFN-γ, IFN-g, If, If2f, Ifg,IFNG,Mouse

Sequence

HGTVIESLES LNNYFNSSGI DVEEKSLFLD IWRNWQKDGD MKILQSQIIS FYLRLFEVLK DNQAISNNIS VIESHLITTF FSNSKAKKDA FMSIAKFEVN NPQVQRQAFN ELIRVVHQLL PESSLRKRKR SRC

Uniprot Accession

Molecular Weight

Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4, containing 5 % trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Background

Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. It shares high sequence indentity with rat IFN-γ (86 %). IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation.

Storage

-20ºC

Research area

Immunology

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Associated products

  • Size

    Code

    Price

  • 100 ug

    CD00410

    $496.00

  • 20 ug

    CD00410

    $176.00

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Lead time: Within two weeks

We are continuously updating our Recombinant proteins catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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