Product name
Recombinant Mouse HB-EGF protein
Code
CD00419
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
Endotoxin
Less than 1 EU/μg of rMuHB-EGF as determined by LAL method.
Synonyms
HB-EGF,AW047313, Dt, Dtr, Dts, Hegf, Hegfl,HBEGF, Mouse
Sequence
DLEGTDLNLF KVAFSSKPQG LATPSKERNG KKKKKGKGLG KKRDPCLRKY KDYCIHGECR YLQEFRTPSC KCLPGYHGHR CHGLTL
Uniprot Accession
Molecular Weight
Approximately 9.8 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, 500 mM NaCl, pH7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. The protein is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Mouse HB-EGF shares about 81 % a.a. sequence identity with human HB-EGF.
Storage
-20ºC
Research area
Immunology
