Product name
Recombinant Mouse G-CSF protein
Code
CD00437
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg.
Endotoxin
Less than 0.1 EU/μg of rMuG-CSF as determined by LAL method.
Synonyms
CSF3,Csf, Csfg, G-CS, G-CSF, MGI-, MGI-IG, Mouse
Sequence
VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA
Uniprot Accession
Molecular Weight
Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, 150 mM NaCl, 0.01 % Tween-20.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNFα and IFN-γ. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL1 or TNF-α activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.
Storage
-20ºC
Research area
Immunology
