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Home > Product > Recombinant proteins > Recombinant Mouse FGF-acidic protein

Recombinant Mouse FGF-acidic protein

Recombinant Mouse FGF-acidic protein

Product Summary

Size: 10 ug

Purity: > 96 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product Summary

Size: 10 ug

Purity: > 96 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product overview

Product name

Recombinant Mouse FGF-acidic protein

Code

CD00451

Biological activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg in the presence of 10 μg/ml of heparin.

Endotoxin

Less than 1 EU/μg of rMuaFGF as determined by LAL method.

Synonyms

FGF-acidic, Mouse

Sequence

FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG PRTHYGQKAI LFLPLPVSSD

Uniprot Accession

Molecular Weight

Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 140 amino acid residues.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Background

Mouse aFGF, encoded by the FGF1 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Mouse aFGF shares 52 % amino acid sequence identity with bFGF. Mouse aFGF shares 96 % amino acid sequence identity with human aFGF, so it exhibits considerable species crossreactivity between Mouse and human aFGF. In mammalian FGF receptor family has 4 members, FGFR1, FGFR2, FGFR3, and FGFR4, and 1, 2, 3 have 2 sub-types “b”, “c”. aFGF can bind and activate all 7 different FGFRs. Affinity between aFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. aFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. aFGF are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion.

Storage

-20ºC

Research area

Immunology

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Associated products

  • Size

    Code

    Price

  • 10 ug

    CD00451

    $176.00

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Lead time: Within two weeks

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