Product name
Recombinant Mouse FGF-9 protein
Code
CD00452
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Endotoxin
Less than 1 EU/μg of rMuFGF-9 as determined by LAL method.
Synonyms
FGF-9, Ek, Eks, Fgf4b,FGF9,Mouse
Sequence
MPLGEVGSYF GVQDAVPFGN VPVLPVDSPV LLNDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY KDILSQS
Uniprot Accession
Molecular Weight
Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 207 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 8.5.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Fibroblast growth factor 9 (FGF-9) encoded by the FGF-9 gene is a member of the fibroblast growth factor (FGF) family. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In addition, this protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells and it is a heparin-binding protein. Furthermore, FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Recombinant mouse FGF-9 is synthesized as a 208 a.a. precursor that contains a 3 a.a. signal sequence. Specifically, The mouse FGF-9 shares 99 % a. a. sequence identity with rat FGF-9.
Storage
-20ºC
Research area
Immunology
