Product name
Recombinant Mouse Exodus-2/CCL21 protein
Code
CD00469
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using Mouse T-lymphocytes is in a concentration range of 10-100 ng/ml.
Endotoxin
Less than 1 EU/μg of rMuExodus-2/CCL21 as determined by LAL method.
Synonyms
Exodus-2/CCL21,6CKBAC, 6CKBAC2, 6Cki, 6Ckine, ALP, AW987545, CKb9, Exodus, Gm1987, SCYA21a, SLC, Scya, Scya2, Scya21, Scya21b, Tca, Tca4, plt, Mouse
Sequence
SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG
Uniprot Accession
Molecular Weight
Approximately 12.1 kDa, a single, non-glycosylated polypeptide chain containing 110 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
CCL21 is encoded by CCL21 gene that is located in Chr. 4 and shows 21-33 % identity to other CC chemokines. This chemokine contains the four conserved cysteines characteristic of β chemokines plus two additional cysteines in its unusually long carboxylterminal domain. Human and mouse CCL21 are highly conserved, exhibiting 86 % amino acid sequence identity. CCL21 is highly expressed in lymph nodes of certain endothelial cells, and in the spleen and appendix. It elicits effects by binding with CCR7, having functions of T and B lymphocytes chemotaxis and inhibiting hematopoiesis.
Storage
-20ºC
Research area
Immunology
