Product name
Recombinant Mouse Eotaxin/CCL11 protein
Code
CD00474
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/ml.
Endotoxin
Less than 1 EU/μg of rMuEotaxin/CCL11 as determined by LAL method.
Synonyms
Eotaxin/CCL11, Scya, Scya11, eot, eotaxin,Mouse
Sequence
HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
Uniprot Accession
Molecular Weight
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
Mouse CCL11 is belonging to the CC chemokine family. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Mouse CCL11 is approximately 63 % identical at the amino acid level to human CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor.
Storage
-20ºC
Research area
Immunology
