Product name
Recombinant Mouse C10/CCL6 protein
Code
CD00604
Biological activity
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR1 transfected Mouse BaF3 cells is in a concentration range of 10-100 ng/ml.
Endotoxin
Less than 1 EU/μg of rMuC10/CCL6 as determined by LAL method.
Synonyms
C10/CCL6,MRP, MRP-1, Scy, Scya6, c10, Mouse
Sequence
GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA
Uniprot Accession
Molecular Weight
Approximately 10.7 kDa, a single non-glycosylated polypeptide chain containing 95 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, pH 8.0, 500 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Chemokine (C-C motif) ligand 6 (CCL6), encoded by the gene CCL6, is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. Mouse CCL6 is expressed in myelopoietic bone marrow cultures when stimulated with GM-CSF, M-CSF, IL-3 or IL-4 and has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. It signals primarily through the CCR1 receptor. CCL6 is chemotactic for B cells, CD4+ T cells, monocytes and NK cells and also exhibits powerful suppressive activity on colony formation by different lineages of hematopoietic progenitors. The C10 contains the four highly conserved cysteine residues present in CC chemokines.
Storage
-20ºC
Research area
Immunology
