Product name
Recombinant Mouse Betacellulin protein
Code
CD00612
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using Mouse Balb/c 3T3 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.
Endotoxin
Less than 1 EU/μg of rMuBetacellulin as determined by LAL method.
Synonyms
Betacellulin, BCN,BTC,Mouse
Sequence
DGNTTRTPET NGSLCGAPGE NCTGTTPRQK VKTHFSRCPK QYKHYCIHGR CRFVVDEQTP SCICEKGYFG ARCERVDLFY
Uniprot Accession
Molecular Weight
Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Betacellulin (BTC) encoded by the BTC gene located on the chromosome 4, is a member of the EGF family that also includes EGF, TGF-alpha, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. Mouse BTC is expressed as a 178-amino acid precursor and the amino acid sequence of the mature form is 80 % identical with human BTC. Both human and mouse BTC exhibit significant overall similarity with other members of the EGF family. Recombinant Mouse Betacellulin is a heparin binding protein containing 80 amino acids residues.
Storage
-20ºC
Research area
Immunology
