Product name
Recombinant Human TAFA-2 protein
Code
CD00852
Biological activity
Fully biologically active when compared to standard. The biological activity is determined by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. rHuTAFA-2, immobilized at 6-24 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Endotoxin
Less than 1 EU/μg of rHuTAFA-2 as determined by LAL method.
Synonyms
TAFA-2,FAM19A2, TAFA-2, Human
Sequence
ANHHKAHHVK TGTCEVVALH RCCNKNKIEE RSQTVKCSCF PGQVAGTTRA APSCVDASIV EQKWWCHMQP CLEGEECKVL PDRKGWSCSS GNKVKTTRVT H
Uniprot Accession
Molecular Weight
Approximately 11.2 kDa, a single, non-glycosylated polypeptide chain containing 101 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
TAFA-2 also named FAM19A2, is a chemokine-like protein which is belonged to the FAM19/TAFA family. The family is a newly discovered and distantly related to MIP-1α. It contains 5 members and TAFA proteins are highly expressed in specific brain regions. Like other members of the TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues. Human TAFA2 is 97 % a.a. identical to mouse TAFA2. The biological functions of TAFA family members remain to be determined, but they are postulated to act as brain-specific chemokines or neurokines that take parts in regulators of immune and nervous cells.
Storage
-20ºC
Research area
Immunology
