Product name
Recombinant Human PTN protein
Code
CD01025
Biological activity
Fully biologically active when compared to standard. The biological activity was measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons, when neurons were plated on 96 well culture plates that had been pre-coated with 100 μl/well of a solution of 5-10 μg/ml rHuPTN.
Endotoxin
Less than 0.1 EU/μg of rHuPTN as determined by LAL method.
Synonyms
PTN, HARP, HB-GAM, HBBM, HBGF-8, HBGF8, HBNF, HBNF-1, NEGF1, OSF-1,Human
Sequence
GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD
Uniprot Accession
Molecular Weight
Approximately 15.3 kDa, a single non-glycosylated polypeptide chain containing 136 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Pleiotrophin and Midkine are structurally related heparin-binding neurotrophic factors, whose expression is developmentally regulated. The expression pattern of these neurotrophic factors suggests function in neurogenesis, cell migration, secondary organogenetic induction, and mesoderm epithelial interaction. The expression of PTN increases during the process of brain embryogenesis and reaches maximum levels at time of birth. The physiological roles of PTN and Midkine are largely unknown, but these neurotrophins have been implicated in the pathogenesis of neuroblastomas. Recombinant human Pleiotrophin is a 15.3 kDa protein containing 136 amino acid residues and 5 intra-molecular disulfide bonds. PTN is a highly conserved protein. The amino acid sequences of human, bovine, rat, and mouse PTN share > 98 % identity.
Storage
-20ºC
Research area
Others
