Product name
Recombinant Human Neuritin protein
Code
CD01229
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by by a cell proliferation assay using rat C6 cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 × 104 IU/mg.
Endotoxin
Less than 0.1 EU/μg of rHuNeuritin as determined by LAL method.
Synonyms
NRN1,NRN, dJ380B8.2,Neuritin, Human
Sequence
AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN
Uniprot Accession
Molecular Weight
Approximately 19.4 kDa, a disulfide-linked homodimeric protein containing two 88 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Neuritin is a neurotrophic factor, which is expressed in response to induction of neuronal activity by NGF, BDNF, NT3, and other neural stimulators. It is expressed primarily in postmitotic-differentiating neurons of the developing nervous system and in neuronal structures related to synaptic plasticity in the adult nervous system. Neuritin acts as a molecular mediator of neurite outgrowth, neuronal survival, and synaptic maturation. Recombinant human Neuritin is a 19.4 kDa, covalently disulfide-linked homodimer, consisting of two polypeptide monomers, each containing 88 amino acid residues.
Storage
-20ºC
Research area
Others
