Product name
Recombinant Human IFN- Alpha1b protein
Code
CD01611
Biological activity
Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0 × 108 IU/mg.
Endotoxin
Less than 1 EU/μg of rHuIFN-α1b as determined by LAL method.
Synonyms
IFN-α1b, IFN-alpha-2, IFN-alphaA, IFNAB, leIF A, IFNA2,IFNA1, Human
Sequence
MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNVDSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE
Uniprot Accession
Molecular Weight
Approximately 19.5 kDa, a single non-glycosylated polypeptide chain containing 167 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4, containing 4 % mannitol and 1 % HSA.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
IFN-αs are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-α family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
Storage
-20ºC
Research area
Immunology
