Product name
Recombinant Human GRO- Alpha/CXCL1 protein
Code
CD01699
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 10-50 ng/ml.
Endotoxin
Less than 1 EU/μg of rHuGRO-α/CXCL1 as determined by LAL method.
Synonyms
GRO-α/CXCL1, FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1,Human
Sequence
ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN
Uniprot Accession
Molecular Weight
Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
CXCL1 is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. The gene for CXCL1 was initially discovered in hamster cells. In addition to the GRO gene, two GRO genes, GROβ and GROγ share 90 % and 86 % amino acid sequence identity with CXCL1/GROα. All three human GROs are members of the intercrine alpha (chemokine C-X-C) subfamily of chemokine. CXCL1 is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing.
Storage
-20ºC
Research area
Immunology
