Product name
Recombinant Human Galectin-1 protein
Code
CD01752
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human blood monocytes is in a concentration range of 1.0-10 ng/ml.
Endotoxin
Less than 0.1 EU/ μg of rHuGalectin -1 as determined by LAL method.
Synonyms
Galectin-1,LGALS1, GAL1, GBP,Human
Sequence
ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD
Uniprot Accession
Molecular Weight
Approximately 14.6 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.
Formulation
Lyophilized from a 0.2 μ m filtered solution in PBS,pH 7.4，with 1 mM DTT.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentrati on of 0.1 - 1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Human Galectin-1 also named BHL, Galaptin, GBP, L- 14 and LGALS1, is belonging to the galectins family and it is encoded by the LGALS1 gene in human. Galectin-1 is expressed by the endometrial stromal cells throughout the menstrual cycle. Galectin-1 contains a single carbohydrate recognition domain through which it can bind glycans both as a monomer and as a homodimer. Dimers are non-covenantly bound and will spontaneou sly disassociate in low concentration. Galectin -1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin - 1 is thought to play a role in the immunosuppression required for a successful pregnancy. Human Galectin-1 shares 88 %-90 % amino acid sequence identity with rat and mouse.
Storage
-20ºC
Research area
Others
