Product name
Recombinant Human ENA-78/CXCL5 protein
Code
CD01874
Biological activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10.0-100.0 ng/ml.
Endotoxin
Less than 1 EU/ug of rHuENA-78, 8 - 78 a.a./CXCL5 as determined by LAL method.
Synonyms
ENA-78, 8-78 a.a./CXCL5,ENA-78, SCYB5, Human
Sequence
LRELRCVCLQ TTQGVHPKMI SNLQVFAIGP QCSKVEVVAS LKNGKEICLD PEAPFLKKVI QKILDGGNKE N
Uniprot Accession
Molecular Weight
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 71 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Background
CXCL5 is a member of the CXC chemokine family and also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. In vitro, ENA-78 (8-78) and ENA-78 (9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. They are produced by proteolytic cleavage after secretion from peripheral blood monocytes. Recombinant human CXCL5 (8-78 a.a.) contains 71 amino acids which is a single non-glycosylated polypeptide chain. Human CXCL5 shares 57 % amino acid sequence identity with mouse and rat CXCL5.
Storage
-20ºC
Research area
Immunology
