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Home > Product > Recombinant proteins > Recombinant Human Angiostatin protein

Recombinant Human Angiostatin protein

Recombinant Human Angiostatin protein

Product Summary

Size: 10 ug

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product Summary

Size: 10 ug

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Source: Escherichia coli.

Product overview

Product name

Recombinant Human Angiostatin protein

Code

CD02341

Biological activity

Fully biologically active when compared to standard. The specific activity determined by an assay on anti-proliferation and anti-migration using endothelial cells in vitro and anti-angiogenesis in vivo is 5.5 × 105 IU/mg.

Endotoxin

Less than 1 EU/μg of rHuAngiostatin as determined by LAL method.

Synonyms

ANG,ALS9, HEL168, RAA1, RNASE4, RNASE5,Angiostatin, Human

Sequence

VYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLK GTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DG

Uniprot Accession

Molecular Weight

Approximately 29.7 KDa, a single non-glycosylated polypeptide chain containing 259 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM NaAc, pH 5.5, 4 % mannitol.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Background

Angiostatin, is a ~30 kDa fragment of plasminogen that is encoded by the PLG gene in humans. It is produced, for example, by autoproteolytic cleavage of plasminogen, involving extracellular disulfide bond reduction by phosphoglycerate kinase. Furthermore, angiostatin can be cleaved from plasminogen by different metalloproteinases (MMPs), elastase, prostate-specific antigen (PSA), 13 kDa serine protease, or 24 kDa endopeptidase. Angiostatin is known to bind many proteins, especially to angiomotin and endothelial cell surface ATP synthase but also integrins, annexin II, C-met receptor, NG2 proteoglycan, tissue-type plasminogen activator, chondroitin sulfate proteoglycans, and CD26. It seems to involve inhibition of endothelial cell migration, proliferation and induction of apoptosis, but its mechanism of action is still unclear. Angiostatin is currently undergoing clinical trials for its use in anticancer therapy. Recombinant angiostatin is expressed in E. coli..

Storage

-20ºC

Research area

Immunology

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Associated products

  • Size

    Code

    Price

  • 10 ug

    CD02341

    $176.00

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Lead time: Within two weeks

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