Active Caspase-3 Monoclonal Antibody(5E1)

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Home > Product > Antibody > Active Caspase-3 Monoclonal Antibody(5E1)

Active Caspase-3 Monoclonal Antibody(5E1)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat, Chicken

Applications: IF, ICC, WB, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat,Chicken

Applications: IF, ICC, WB, IHC-p

Product overview

Full product name

Caspase-3

Code

BT-MCA0002

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Recombinant Protein of Active Caspase-3

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

N/A

Gene ID

Human: 836

Synonyms

CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Research area

Cell Biology; Metabolism; Cancer

Target protein

CASP3

Recommended dilution

Application	Dilution
IF	1:50-200
IHC	1:100-200
WB	1:500-1000

Images

Immunofluorescence analysis of Hela cell. FoxO1 (phospho Ser256) Polyclonal Antibody(red) was diluted at 1:200(4°C overnight). Active Caspase-3 Monoclonal antibody(5E1)(green) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of rat-lung tissue. 1.Active Caspase-3 Monoclonal antibody(5E1)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Active Caspase-3 Monoclonal antibody(5E1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Active Caspase-3 Monoclonal antibody(5E1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1.Active Caspase-3 Monoclonal antibody(5E1) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Western blot analysis of 1) Hela, 2) 3T3. 3) Rat Brain Tissue using Active Caspase-3 Monoclonal antibody.

Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using Active Caspase-3 Monoclonal antibody.

Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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